INVERTASE 200,000

INTRODUCTION

Invertase is a yeast-derived enzyme. Invertase splits sucrose into glucose and fructose (invert syrup) and can be applied for any inversion of sucrose especially liquefied cherry centers, creams, mints, truffles, marshmallow, invert syrup and other fondants. Invertase is used to improve shelf life of confections. It is available in single, double and triple strengths and is packaged in one, ten and 44 pound containers for ease of use, storage and cost efficiency. Custom packaging is also available.

Invertase 200,000 is powdered invertase preparation which can be applied in the confectionary industry for the production of invert syrup starting from beet or cane sugar. When  Invertase 200,000 is used for this process, no browning appears and no H.M.F. (hydroxy-methylfurfural) will form, which is a normal phenomenon during hydrolysis with acid. Invertase can also be used to prevent sugar crystallization in confections by hydrolysis of sucrose (glucose + fructose) in fondants or chocolate coated candies with soft centers.

In addition to its main confectionary application, Invertase 200,000 can be used to produce melibiose from raffinose or D-Fructose from inulin, since it contains b-fructosidase activity. This enzyme can also be used in some specialty fruit juice products to decrease sucrose levels.

Invertase 200,000 is derived from a selected non-GMO yeast strain of Saccharomyces cerevisiae.

SPECIFICATIONS 

The activity of Invertase 200,000 may be determined by taking advantage of the fact that invert sugar is a reducing sugar,
while sucrose is not. The activity of invertase can be valued by measuring the progress of the reduction of a sucrose solution,
being hydrolyzed by invertase under fixed standard conditions over a certain time interval. This activity is expressed in S.U.
(Sumner Units). One S.U. is that quantity of enzyme which forms 1 mg of invert sugar out of 6 ml 5.4% sucrose solution under
fixed conditions at 20°C and pH 4.7 in 5 minutes. Invertase 200,000 is standardized at 200,000 S.U. per gram.
1 S.U. = 0.3279 INVU.

DOSAGE 

70% sucrose syrup can be completely hydrolyzed by Invertase 200,000 without any difficulty. To be sure of this, the syrup
must be thoroughly mixed with an adequate amount of enzyme and kept sufficiently long at the desired temperatures, i.e., at
50°C. It is not necessary to take further measures. It can be proved by experiments that 20,000—50,000 SU/kg of dry matter, depending upon temperature and time of reaction (12 - 24 hours at 50°C), are sufficient for a complete hydrolysis of
70% sugar syrup. Considerably shorter reaction times may be reached using larger quantities of enzyme; whereas, with
longer times of reaction, a much smaller quantity of enzyme will suffice. For inulin hydrolysis 5,000—10,000 SU/kg dry matter
should be used. In apple juice, 1000 SU/liter is recommended.

PACKAGING AND STORAGE

Invertase 200,000 is packaged in 44-pound fiber drums. It should be stored in a cool, dry location.

CHARACTERISTICS

Appearance:            Light tan color
Solubility:                 Miscible with water
pH:                          5.3 - 5.6

OPTIMUM TEMPERATURE

The optimum temperature for invertase is 140° F (60°C). The enzyme has excellent activity at lower temperatures, but reaction times are extended. To compensate for the extended reaction times, more enzyme may be used. For ideal results, each application should be optimized.

TEMPERATURE STABILITY

Invertase can be used at temperatures up to 140° F (60° C) without loss of activity. Above 140° F activity begins to decrease; however, more enzyme may be used to compensate for loss of activity. It may be necessary to optimize applications requiring higher temperatures depending on actual temperatures.

SAFE HANDLING

Invertase and all chemicals should be handled properly. Invertase is nonflammable. All invertase preparations can be allergens and cause sensitivities on prolonged contact. Gloves and safety glasses should be used when handling this material to prevent repeated skin or eye contact. Repeated exposure can lead to allergic sensitization. Should this material come in contact with skin or eyes, rinse thoroughly for 10 to 15 minutes with water. For eye contact, rinse thoroughly for 10 to 15 minutes with water and seek medical attention. If any symptoms such as skin rashes or respiratory difficulties persist, seek prompt medical attention.

A Material Safety Data Sheet is available upon request.

Disclaimer

The information provided above is to the best of our knowledge is true and accurate. Recommendations and suggestions are made without warranty or guarantee because conditions of use are not within our control.

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